Difference between revisions of "RbgA"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
+
* essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+
* depletion confers a slow growth phenotype, and induces an abnormality of the ''in vivo'' ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes
 
+
* premature 45S particles accumulated in YlqF-depleted cells lack several proteins, including [[rplP|L16]], [[rpmA|L27]] and [[rpmJ|L36]]
 
=== Database entries ===
 
=== Database entries ===
  
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==Original publications==
 
==Original publications==
'''Additional publications:''' {{PubMed|22267738}}
+
<pubmed>12427945, 16390447 16997968 23325847 22267738 23700310 </pubmed>
<pubmed>12427945, 16390447 16997968 23325847 </pubmed>
 
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:28, 12 September 2013

  • Description: assembly of the 50S subunit of the ribosome

Gene name rbgA
Synonyms ylqF
Essential yes PubMed
Product GTPase
Function ribosome assembly
Gene expression levels in SubtiExpress: rbgA
MW, pI 31 kDa, 9.528
Gene length, protein length 846 bp, 282 aa
Immediate neighbours rplS, rnhB
Sequences Protein DNA DNA_with_flanks
Genetic context
YlqF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RbgA expression.png















Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16050

Phenotypes of a mutant

  • essential PubMed
  • depletion confers a slow growth phenotype, and induces an abnormality of the in vivo ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes
  • premature 45S particles accumulated in YlqF-depleted cells lack several proteins, including L16, L27 and L36

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: MTG1 subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
    • RNA-binding domain at the C-terminus PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)


Original publications