Difference between revisions of "TcyN"

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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 777 bp, 259 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 777 bp, 259 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytmO]]'', ''[[tcyM]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cmoO]]'', ''[[tcyM]]''
 
|-
 
|-
 
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29340 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29340 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29340 DNA_with_flanks]
 
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29340 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29340 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29340 DNA_with_flanks]
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 
{{SubtiWiki regulon|[[CymR regulon]]}},
 
{{SubtiWiki regulon|[[CymR regulon]]}},
{{SubtiWiki regulon|[[YtlI regulon]]}}
+
{{SubtiWiki regulon|[[AscR regulon]]}}
  
 
=The gene=
 
=The gene=
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=Expression and regulation=
 
=Expression and regulation=
* '''Operon:''' ''[[ytmI]]-[[tcyJ]]-[[tcyK]]-[[tcyL]]-[[tcyM]]-[[tcyN]]-[[ytmO]]-[[ytnI]]-[[ytnJ]]-[[ribR]]-[[ytnL]]-[[ytnM]]'' {{PubMed|15668000}}
+
* '''Operon:''' ''[[snaA]]-[[tcyJ]]-[[tcyK]]-[[tcyL]]-[[tcyM]]-[[tcyN]]-[[cmoO]]-[[cmoI]]-[[cmoJ]]-[[ribR]]-[[sndA]]-[[ytnM]]'' {{PubMed|15668000}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tcyN_3004346_3005125_-1 tcyN] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tcyN_3004346_3005125_-1 tcyN] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|15272571}}
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|15272571}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 
** repressed in the presence of cysteine ([[CymR]]) {{PubMed|16109943}}
 
** repressed in the presence of cysteine ([[CymR]]) {{PubMed|16109943}}
 +
** induced in the presence of S-methyl-cysteine ([[AscR]]) {{PubMed|23944997}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[CymR]]: transcription repression {{PubMed|16109943}}
 
** [[CymR]]: transcription repression {{PubMed|16109943}}
** [[YtlI]]: transcription activation {{PubMed|16109943}}  
+
** [[AscR]]: transcription activation {{PubMed|16109943}}  
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
 
+
<pubmed>15262924,10092453,16109943,12193636, 15668000 15272571 23944997</pubmed>
<pubmed>15262924,10092453,16109943,12193636, 15668000</pubmed>
 
'''Additional publications:''' {{PubMed|15272571}}
 
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:23, 18 August 2013

Gene name tcyN
Synonyms hisP, ytmN
Essential no
Product cystine ABC transporter (ATP-binding protein)
Function cystine uptake
Gene expression levels in SubtiExpress: tcyN
Interactions involving this protein in SubtInteract: TcyN
Metabolic function and regulation of this protein in SubtiPathways:
Riboflavin / FAD
MW, pI 29 kDa, 8.835
Gene length, protein length 777 bp, 259 aa
Immediate neighbours cmoO, tcyM
Sequences Protein DNA DNA_with_flanks
Genetic context
YtmN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TcyN expression.png















Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CymR regulon, AscR regulon

The gene

Basic information

  • Locus tag: BSU29340

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2Q0H (complex with ADP-Mg2+, Geobacillus stearothermophilus, 54% identity )
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of cysteine (CymR) PubMed
    • induced in the presence of S-methyl-cysteine (AscR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Che-Man Chan, Antoine Danchin, Philippe Marlière, Agnieszka Sekowska
Paralogous metabolism: S-alkyl-cysteine degradation in Bacillus subtilis.
Environ Microbiol: 2014, 16(1);101-17
[PubMed:23944997] [WorldCat.org] [DOI] (I p)

Pierre Burguière, Juliette Fert, Isabelle Guillouard, Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism.
J Bacteriol: 2005, 187(17);6019-30
[PubMed:16109943] [WorldCat.org] [DOI] (P p)

Irina M Solovieva, Rimma A Kreneva, Lubov Errais Lopes, Daniel A Perumov
The riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of a 10 kb operon, which is negatively regulated by the yrzC gene product.
FEMS Microbiol Lett: 2005, 243(1);51-8
[PubMed:15668000] [WorldCat.org] [DOI] (P p)

I M Solov'eva, R A Kreneva, L L Erraĭs, A S Mironov, D A Perumov
[Study of the mechanism for regulating ribR gene activity in Bacillus subtilis]. [Issledovanie mekhanizma reguliatsii aktivnosti gena ribR u Bacillus subtilis.]
Genetika: 2004, 40(5);716-20
[PubMed:15272571] [WorldCat.org] (P p)

Pierre Burguière, Sandrine Auger, Marie-Françoise Hullo, Antoine Danchin, Isabelle Martin-Verstraete
Three different systems participate in L-cystine uptake in Bacillus subtilis.
J Bacteriol: 2004, 186(15);4875-84
[PubMed:15262924] [WorldCat.org] [DOI] (P p)

Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Global expression profile of Bacillus subtilis grown in the presence of sulfate or methionine.
J Bacteriol: 2002, 184(18);5179-86
[PubMed:12193636] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)