Difference between revisions of "LigD"

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(Biological materials)
(Biological materials)
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* '''Mutant:'''  
 
* '''Mutant:'''  
 
** BP141 (''[[ykoVU]]''::''kan'') available in [[Fabian Commichau]]'s lab
 
** BP141 (''[[ykoVU]]''::''kan'') available in [[Fabian Commichau]]'s lab
 +
** BP142 (''[[ykoWVU]]''::''kan'') available in [[Fabian Commichau]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:39, 6 August 2013

  • Description: DNA repair polymerase/ ligase in non-homologous end joining DNA repair

Gene name ligD
Synonyms ykoU
Essential no
Product DNA repair polymerase/ ligase
Function non-homologous end joining DNA repair, repair of gapped DNA substrates
Gene expression levels in SubtiExpress: ligD
Interactions involving this protein in SubtInteract: LigD
MW, pI 70 kDa, 6.646
Gene length, protein length 1833 bp, 611 aa
Immediate neighbours ykoT, ykoV
Sequences Protein DNA DNA_with_flanks
Genetic context
YkoU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkoU expression.png















Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon


The gene

Basic information

  • Locus tag: BSU13400

Phenotypes of a mutant

  • sensitivity to ionizing radiation in the stationary phase PubMed
  • sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: N-terminal DNA ligase catalytic domain (aa 1 - 331) linked to a C-terminal polymerase domain (aa 332 - 611) PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Stewart Shuman, Michael S Glickman
Bacterial DNA repair by non-homologous end joining.
Nat Rev Microbiol: 2007, 5(11);852-61
[PubMed:17938628] [WorldCat.org] [DOI] (I p)

Original publications