SpeD

• Description: S-adenosylmethionine decarboxylase
  
  

Gene name	speD
Synonyms	ytcF
Essential	no
Product	S-adenosylmethionine decarboxylase
Function	spermidine, polyamine biosynthesis
Gene expression levels in SubtiExpress: speD
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation, Central C-metabolism
MW, pI	13 kDa, 4.768
Gene length, protein length	384 bp, 128 aa
Immediate neighbours	ytcG, gapB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways

This gene is a member of the following regulons

CcpN regulon

The gene

Basic information

• Locus tag: BSU29010

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-adenosyl-L-methionine = (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt + CO₂ (according to Swiss-Prot)

• Protein family: Type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1VR7 (from Thermotoga maritima, 47% identity, 72% similarity)

• UniProt: O34426

• KEGG entry: [3]

• E.C. number: 4.1.1.50

Expression and regulation

• Operon:
  • gapB-speD PubMed
  • speD PubMed

• Expression browser: speD PubMed

• Sigma factor:
  • gapB: SigA PubMed
  • speD: SigA PubMed

• Regulation:
  • gapB: repressed in the presence of glucose (CcpN) PubMed

• Regulatory mechanism:
  • CcpN: transcription repression PubMed

• Additional information:
  • speD: the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic speD mRNA increases from 1.4 to 36 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947

Pascale Servant, Dominique Le Coq, Stéphane Aymerich
CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes.
Mol Microbiol: 2005, 55(5);1435-51
[PubMed:15720552] [WorldCat.org] [DOI] (P p)

A Sekowska, J Y Coppée, J P Le Caer, I Martin-Verstraete, A Danchin
S-adenosylmethionine decarboxylase of Bacillus subtilis is closely related to archaebacterial counterparts.
Mol Microbiol: 2000, 36(5);1135-47
[PubMed:10844697] [WorldCat.org] [DOI] (P p)

A Sekowska, P Bertin, A Danchin
Characterization of polyamine synthesis pathway in Bacillus subtilis 168.
Mol Microbiol: 1998, 29(3);851-8
[PubMed:9723923] [WorldCat.org] [DOI] (P p)