Difference between revisions of "Csn"

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Revision as of 14:02, 16 May 2013

  • Description: chitosanase

Gene name csn
Synonyms
Essential no
Product chitosanase
Function chitin degradation
Gene expression levels in SubtiExpress: csn
MW, pI 31 kDa, 8.892
Gene length, protein length 831 bp, 277 aa
Immediate neighbours yraM, yraL
Sequences Protein DNA DNA_with_flanks
Genetic context
Csn context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Csn expression.png
























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

  • Locus tag: BSU26890

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 46 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Chulhong Oh, Mahanama De Zoysa, Do-Hyung Kang, Youngdeuk Lee, Ilson Whang, Chamilani Nikapitiya, Soo-Jin Heo, Kon-Tak Yoon, Abu Affan, Jehee Lee
Isolation, purification, and enzymatic characterization of extracellular chitosanase from marine bacterium Bacillus subtilis CH2.
J Microbiol Biotechnol: 2011, 21(10);1021-5
[PubMed:22031025] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Luis A Rivas, Vı Ctor Parro, Mercedes Moreno-Paz, Rafael P Mellado
The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a streptomyces enzyme.
Microbiology (Reading): 2000, 146 ( Pt 11);2929-2936
[PubMed:11065371] [WorldCat.org] [DOI] (P p)