Difference between revisions of "ProI"
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Revision as of 13:50, 16 May 2013
- Description: pyrroline-5-carboxylate reductase
| Gene name | proI |
| Synonyms | yqjO |
| Essential | no |
| Product | pyrroline-5-carboxylate reductase |
| Function | biosynthesis of proline |
| Gene expression levels in SubtiExpress: proI | |
| Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress | |
| MW, pI | 30 kDa, 6.702 |
| Gene length, protein length | 834 bp, 278 aa |
| Immediate neighbours | yqjP, yqjN |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23800
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)
- Protein family: pyrroline-5-carboxylate reductase family (according to Swiss-Prot)
- Paralogous protein(s): ProH
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P54552
- KEGG entry: [3]
- E.C. number: 1.5.1.2
Additional information
Expression and regulation
- Operon: proI PubMed
- Regulatory mechanism:
- T-box: RNA switch, transcriptional antitermination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Jeanette Brill, Tamara Hoffmann, Harald Putzer, Erhard Bremer
T-box-mediated control of the anabolic proline biosynthetic genes of Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 4);977-987
[PubMed:21233158]
[WorldCat.org]
[DOI]
(I p)
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
B R Belitsky, J Brill, E Bremer, A L Sonenshein
Multiple genes for the last step of proline biosynthesis in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4389-92
[PubMed:11418582]
[WorldCat.org]
[DOI]
(P p)
