Difference between revisions of "PrkD"
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| − | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ybdM_223219_223989_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:prkD_expression.png|500px]] | + | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ybdM_223219_223989_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:prkD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02030]] |
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Revision as of 12:26, 16 May 2013
- Description: protein kinase D
| Gene name | prkD |
| Synonyms | ybdM |
| Essential | no |
| Product | protein kinase D |
| Function | unknown |
| Gene expression levels in SubtiExpress: prkD | |
| MW, pI | 29 kDa, 6.359 |
| Gene length, protein length | 768 bp, 256 aa |
| Immediate neighbours | ybzI, ybdN |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
protein modification, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU02030
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: protein kinase domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: autophosphorylated on Thr-174 (in vitro) PubMed
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O31435
- KEGG entry: [2]
- E.C. number: 2.7.11.1
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP578 (aphA3), available in the Stülke lab
- Expression vector:
- pGP390 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP821, available in Stülke lab
- for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1407, available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
