Difference between revisions of "Crh"

From SubtiWiki
Jump to: navigation, search
Line 26: Line 26:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvcN]]'', ''[[yvcL]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvcN]]'', ''[[yvcL]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34740 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34740 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34740 Advanced_DNA]
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34740 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34740 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34740 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:crh_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:crh_context.gif]]

Revision as of 11:21, 14 May 2013

  • Description: "Carbon-flux regulating HPr", formerly known as "Catabolite repression HPr-like protein", control of flux through the harmful methylglyoxal pathway, minor cofactor of the CcpA transcription factor

Gene name crh
Synonyms yvcM
Essential no
Product carbon-flux regulating HPr
Function control of carbon flux
Gene expression levels in SubtiExpress: crh
Interactions involving this protein in SubtInteract: Crh
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 9,2 kDa, 4.70
Gene length, protein length 255 bp, 85 amino acids
Immediate neighbours yvcN, yvcL
Sequences Protein DNA DNA_with_flanks
Genetic context
Crh context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Crh expression.png




























Categories containing this gene/protein

transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU34740

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: HPr family
  • Paralogous protein(s): HPr

Extended information on the protein

  • Kinetic information:
  • Domains: HPr domain (1–85)
  • Modification: phosphorylation on Ser46 by HprK PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries


  • KEGG entry: [2]

Additional information

Crh does not possess the phosphorylation site used for PTS phosphotransfer (His-15 in PtsH), it can only be phosphorylated on Ser-46

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information: Crh is weakly expressed. This results in part from a poorly conserved ribosomal binding site of the mRNA. PubMed

Biological materials

  • Mutant: GP860 (aphA3), QB7097 (spc), available in Stülke lab
  • Expression vector:
    • pGP641 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • pGP734 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab
  • lacZ fusion: see yvcI
  • GFP fusion:
  • two-hybrid system: crh, crh(Ser46Asp), crh(Ser46Ala) B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab


Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Wolfgang Hillen, Erlangen University, Germany Homepage

Richard Brennan, Houston, Texas, USA Homepage

Your additional remarks

References