Crh

• Description: "Carbon-flux regulating HPr", formerly known as "Catabolite repression HPr-like protein", control of flux through the harmful methylglyoxal pathway, minor cofactor of the CcpA transcription factor
  
  

Gene name	crh
Synonyms	yvcM
Essential	no
Product	carbon-flux regulating HPr
Function	control of carbon flux
Gene expression levels in SubtiExpress: crh
Interactions involving this protein in SubtInteract: Crh
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	9,2 kDa, 4.70
Gene length, protein length	255 bp, 85 amino acids
Immediate neighbours	yvcN, yvcL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, phosphoproteins

The gene

Basic information

• Locus tag: BSU34740

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: HPr family

• Paralogous protein(s): HPr

Extended information on the protein

• Kinetic information:

• Domains: HPr domain (1–85)

• Modification: phosphorylation on Ser46 by HprK PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Crh-GapA PubMed
  • Crh-CcpA PubMed
  • HprK-Crh PubMed
  • Crh-MgsA (only non-phosphorylated Crh interacts with MgsA) PubMed

• Localization:

Database entries

• Structure:
  • 2AK7 (dimeric Crh-Ser46-P)
  • 1ZVV (CcpA-Crh-DNA complex)
  • 2RLZ (dimer)
  • 1MU4
  • NCBI, dimer
  • NCBI, CcpA-Crh-DNA complex
  • NCBI, dimeric phosphor-Crh
  • NCBI
• UniProt: O06976

• KEGG entry: [2]

Additional information

Crh does not possess the phosphorylation site used for PTS phosphotransfer (His-15 in PtsH), it can only be phosphorylated on Ser-46

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Expression browser: crh PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information: Crh is weakly expressed. This results in part from a poorly conserved ribosomal binding site of the mRNA. PubMed

Biological materials

• Mutant: GP860 (aphA3), QB7097 (spc), available in Stülke lab

• Expression vector:
  • pGP641 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • pGP734 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab

• lacZ fusion: see yvcI

• GFP fusion:

• two-hybrid system: crh, crh(Ser46Asp), crh(Ser46Ala) B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• FLAG-tag construct:
  • GP69 (spc, based on pGP1331), available in the Boris Görke and Stülke labs
  • pGP1200 crh(Ser46Ala) (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Wolfgang Hillen, Erlangen University, Germany Homepage

Richard Brennan, Houston, Texas, USA Homepage