XylA

• Description: xylose isomerase
  
  

Gene name	xylA
Synonyms
Essential	no
Product	xylose isomerase
Function	utilization of xylan and xylose
Gene expression levels in SubtiExpress: xylA
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	50 kDa, 5.635
Gene length, protein length	1335 bp, 445 aa
Immediate neighbours	xylR, xylB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, XylR regulon

The gene

Basic information

• Locus tag: BSU17600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-xylose = D-xylulose (according to Swiss-Prot)

• Protein family: xylose isomerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1A0D (Geobacillus stearothermophilus)

• UniProt: P04788

• KEGG entry: [3]

• E.C. number: 5.3.1.5

Expression and regulation

• Operon: xylA-xylB PubMed

• Expression browser: xylA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • induction by xylose (XylR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • XylR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1151 (del aphA3) available in Stülke lab
  • 1A719 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Hillen, Erlangen University, Germany Homepage