Difference between revisions of "McsA"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ctsR]]'', ''[[mcsB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ctsR]]'', ''[[mcsB]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00840 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00840 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:mcsA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mcsA_context.gif]]

Revision as of 09:14, 14 May 2013

  • Description: modulator of CtsR-dependent repression, McsA activates kinase activity of McsB

Gene name mcsA
Synonyms yacH
Essential no
Product activator of McsB kinase activity
Function control of CtsR activity
Gene expression levels in SubtiExpress: mcsA
Interactions involving this protein in SubtInteract: McsA
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 20 kDa, 6.624
Gene length, protein length 555 bp, 185 aa
Immediate neighbours ctsR, mcsB
Sequences Protein DNA DNA_with_flanks
Genetic context
McsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
McsA expression.png




























Categories containing this gene/protein

transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), heat shock proteins, phosphoproteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon, SigF regulon

The gene

Basic information

  • Locus tag: BSU00840

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-169 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: mcsA::aphA3 available from the Gerth lab
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody: available in Gerth lab

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed