Difference between revisions of "TetB"
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to tetracyclin | |style="background:#ABCDEF;" align="center"|'''Function''' || resistance to tetracyclin | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40770 tetB] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 9.537 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 9.537 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yyaP]]'', ''[[tetL]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yyaP]]'', ''[[tetL]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40770 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:tetB_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:tetB_context.gif]] | ||
Revision as of 14:20, 13 May 2013
- Description: multifunctional tetracycline/Na+ resistance protein
| Gene name | tetB |
| Synonyms | tetA, tetA(L) |
| Essential | no |
| Product | multifunctional tetracycline/Na+ resistance protein |
| Function | resistance to tetracyclin |
| Gene expression levels in SubtiExpress: tetB | |
| MW, pI | 49 kDa, 9.537 |
| Gene length, protein length | 1374 bp, 458 aa |
| Immediate neighbours | yyaP, tetL |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
transporters/ other, resistance against toxins/ antibiotics, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU40770
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: TCR/tet family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P23054
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Your additional remarks
References
W Wang, A A Guffanti, Y Wei, M Ito, T A Krulwich
Two types of Bacillus subtilis tetA(L) deletion strains reveal the physiological importance of TetA(L) in K(+) acquisition as well as in Na(+), alkali, and tetracycline resistance.
J Bacteriol: 2000, 182(8);2088-95
[PubMed:10735849]
[WorldCat.org]
[DOI]
(P p)
S J Stasinopoulos, G A Farr, D H Bechhofer
Bacillus subtilis tetA(L) gene expression: evidence for regulation by translational reinitiation.
Mol Microbiol: 1998, 30(5);923-32
[PubMed:9988470]
[WorldCat.org]
[DOI]
(P p)
J Cheng, D B Hicks, T A Krulwich
The purified Bacillus subtilis tetracycline efflux protein TetA(L) reconstitutes both tetracycline-cobalt/H+ and Na+(K+)/H+ exchange.
Proc Natl Acad Sci U S A: 1996, 93(25);14446-51
[PubMed:8962071]
[WorldCat.org]
[DOI]
(P p)
J Cheng, A A Guffanti, W Wang, T A Krulwich, D H Bechhofer
Chromosomal tetA(L) gene of Bacillus subtilis: regulation of expression and physiology of a tetA(L) deletion strain.
J Bacteriol: 1996, 178(10);2853-60
[PubMed:8631673]
[WorldCat.org]
[DOI]
(P p)
J Cheng, K Baldwin, A A Guffanti, T A Krulwich
Na+/H+ antiport activity conferred by Bacillus subtilis tetA(L), a 5' truncation product of tetA(L), and related plasmid genes upon Escherichia coli.
Antimicrob Agents Chemother: 1996, 40(4);852-7
[PubMed:8849239]
[WorldCat.org]
[DOI]
(P p)
R Sakaguchi, H Amano, K Shishido
Nucleotide sequence homology of the tetracycline-resistance determinant naturally maintained in Bacillus subtilis Marburg 168 chromosome and the tetracycline-resistance gene of B. subtilis plasmid pNS1981.
Biochim Biophys Acta: 1988, 950(3);441-4
[PubMed:2844262]
[WorldCat.org]
[DOI]
(P p)
