Difference between revisions of "MaeN"
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|style="background:#ABCDEF;" align="center"|'''Function''' || malate uptake | |style="background:#ABCDEF;" align="center"|'''Function''' || malate uptake | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31580 maeN] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nupQ]]'', ''[[yufS]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nupQ]]'', ''[[yufS]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31580 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31580 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31580 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:maeN_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:maeN_context.gif]] | ||
Revision as of 13:44, 13 May 2013
- Description: Na+/malate symporter
| Gene name | maeN |
| Synonyms | yufR |
| Essential | no |
| Product | Na+/malate symporter |
| Function | malate uptake |
| Gene expression levels in SubtiExpress: maeN | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 47 kDa, 8.411 |
| Gene length, protein length | 1344 bp, 448 aa |
| Immediate neighbours | nupQ, yufS |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
transporters/ other, utilization of specific carbon sources, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31580
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family
- Paralogous protein(s): CimH
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O05256
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation: induced by malate (MalR)
- Regulatory mechanism: MalR: transcription activation
Biological materials
- Mutant:
- 1A642 (maeN::Tn917, erm) (available from the BGSC or in Jörg Stülke's lab)
- 1A642 ( maeN::erm), PubMed, available at BGSC
- GP1449 (erm), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Iwona Sobczak, Juke S Lolkema
The 2-hydroxycarboxylate transporter family: physiology, structure, and mechanism.
Microbiol Mol Biol Rev: 2005, 69(4);665-95
[PubMed:16339740]
[WorldCat.org]
[DOI]
(P p)
Original publications
Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. Mol Microbiol. 2011 81(6): 1459-1473. PubMed:21815947
Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206]
[WorldCat.org]
[DOI]
(I p)
Kousei Tanaka, Kazuo Kobayashi, Naotake Ogasawara
The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium.
Microbiology (Reading): 2003, 149(Pt 9);2317-2329
[PubMed:12949159]
[WorldCat.org]
[DOI]
(P p)
