Difference between revisions of "AckA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || overflow metabolism
 
|style="background:#ABCDEF;" align="center"|'''Function''' || overflow metabolism
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU29470 ackA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29470 ackA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[moaB]]'', ''[[ytxK]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[moaB]]'', ''[[ytxK]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14925&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29470 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29470 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29470 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ackA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ackA_context.gif]]

Revision as of 13:36, 13 May 2013

  • Description: acetate kinase

Gene name ackA
Synonyms
Essential no
Product acetate kinase
Function overflow metabolism
Gene expression levels in SubtiExpress: ackA
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 42 kDa, 5.191
Gene length, protein length 1185 bp, 395 aa
Immediate neighbours moaB, ytxK
Sequences Protein DNA Advanced_DNA
Genetic context
AckA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AckA expression.png
















Categories containing this gene/protein

ATP synthesis, carbon core metabolism

This gene is a member of the following regulons

CcpA regulon, CodY regulon

The gene

Basic information

  • Locus tag: BSU29470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + acetate = ADP + acetyl phosphate (according to Swiss-Prot)
  • Protein family: acetokinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2IIR (from Thermotoga maritima, 54% identity, 73% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expression activated by glucose (2.5 fold) (CcpA) PubMed
    • activated during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Your additional remarks

References

Reviews

Cheryl Ingram-Smith, Stephen R Martin, Kerry S Smith
Acetate kinase: not just a bacterial enzyme.
Trends Microbiol: 2006, 14(6);249-53
[PubMed:16678422] [WorldCat.org] [DOI] (P p)

Alan J Wolfe
The acetate switch.
Microbiol Mol Biol Rev: 2005, 69(1);12-50
[PubMed:15755952] [WorldCat.org] [DOI] (P p)

Original publications