Difference between revisions of "CitZ"

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|style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
 
|style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU29140 citZ]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29140 citZ]
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CitZ CitZ]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CitZ CitZ]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[icd]], [[ytwI]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[icd]], [[ytwI]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14874&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29140 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29140 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29140 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:citZ_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:citZ_context.gif]]

Revision as of 13:34, 13 May 2013

  • Description: citrate synthase

Gene name citZ
Synonyms citA2
Essential no
Product citrate synthase II
Function TCA cycle
Gene expression levels in SubtiExpress: citZ
Interactions involving this protein in SubtInteract: CitZ
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 41 kDa, 5.451
Gene length, protein length 1116 bp, 372 aa
Immediate neighbours icd, ytwI
Sequences Protein DNA Advanced_DNA
Genetic context
CitZ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CitZ expression.png















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, CcpC regulon

The gene

Basic information

  • Locus tag: BSU29140

Phenotypes of a mutant

glutamate auxotrophy and a defect in sporulation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + H2O + oxaloacetate = citrate + CoA (according to Swiss-Prot)
  • Protein family: citrate synthase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information: Michaelis-Menten (Random Sequential Reaction Mechanism) PubMed
  • Domains:
  • Modification: phosphorylation on Ser-284 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
    • Inhibited by acetyl-CoA, 2-oxoglutarate and NADH PubMed FEBS Letters
    • Inhibited by citrate and CoA (competitively against acetyl-CoA and non-competitively against oxaloacetate) PubMed
    • Inhibited by ATP competitively in B. subtilis strain 168 and HS 1A17 PubMed PubMed
      • In B. subtilis strain HS 2A2, ATP inhibits a non-competitive fashion PubMed
    • Activated by AMP PubMed

Database entries

  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of CitZ can be found at Proteopedia

Expression and regulation

  • Regulation:
    • repression by glucose (6.7-fold) (CcpA) PubMed
    • repression by glucose + glutamate (CcpC) PubMed
    • reduced expression at excess citrate concentrations or iron depletion (CitB) PubMed
  • Regulatory mechanism:
    • CcpA: transcription repression, CcpC: transcription repression PubMed
    • CcpC: transcription repression (molecular inducer: citrate) PubMed
    • CitB: mRNA destabilization upon citrate accumulation or iron limitation PubMed
  • Additional information:
    • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

  • Mutant: GP678 (erm), available in Stülke lab
  • Expression vector:
    • pGP1120 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)
    • pGP1776 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Stülke lab)
    • pGP1761 (expression with N-terminal His-tag from E. coli, in pWH844), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

Reviews

Original publications

Additional publications: PubMed