Difference between revisions of "PheT"

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|style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]]  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]]  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU28630 pheT]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28630 pheT]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PheT PheT]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PheT PheT]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnhC]]'', ''[[pheS]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnhC]]'', ''[[pheS]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14823&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28630 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28630 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28630 Advanced_DNA]
 
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Revision as of 13:32, 13 May 2013

  • Description: phenylalanyl-tRNA synthetase (beta subunit)

Gene name pheT
Synonyms
Essential yes PubMed
Product phenylalanyl-tRNA synthetase (beta subunit)
Function translation
Gene expression levels in SubtiExpress: pheT
Interactions involving this protein in SubtInteract: PheT
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 87 kDa, 4.864
Gene length, protein length 2412 bp, 804 aa
Immediate neighbours rnhC, pheS
Sequences Protein DNA Advanced_DNA
Genetic context
PheT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PheT expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU28630

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)
  • Protein family: Type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1PYS (from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701] [WorldCat.org] [DOI] (P p)