Difference between revisions of "Rph"

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|style="background:#ABCDEF;" align="center"|'''Function''' || 3'-5' exoribonuclease  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || 3'-5' exoribonuclease  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU28370 rph]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28370 rph]
 
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|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 4.888   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 4.888   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ysnA]]'', ''[[gerM]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ysnA]]'', ''[[gerM]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14797&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28370 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28370 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28370 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:rph_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:rph_context.gif]]

Revision as of 13:31, 13 May 2013

  • Description: RNase PH, 3'-5' exoribonuclease

Gene name rph
Synonyms
Essential no
Product RNase PH (EC 2.7.7.56)
Function 3'-5' exoribonuclease
Gene expression levels in SubtiExpress: rph
MW, pI 26 kDa, 4.888
Gene length, protein length 735 bp, 245 aa
Immediate neighbours ysnA, gerM
Sequences Protein DNA Advanced_DNA
Genetic context
Rph context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Rph expression.png
























Categories containing this gene/protein

Rnases, translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU28370

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • major player in the exonucleolytic maturation of CCA-containing tRNA precursors PubMed
    • responsible for endonucleolytic cleavage of scRNA (scr) PubMed
  • Protein family: RNase PH family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

Your additional remarks

References