Difference between revisions of "QueA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification  
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU27720 queA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27720 queA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 38 kDa, 5.047   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 38 kDa, 5.047   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tgt]]'', ''[[yrzS]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tgt]]'', ''[[yrzS]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14732&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27720 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27720 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27720 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:queA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:queA_context.gif]]

Revision as of 13:27, 13 May 2013

  • Description: S-adenosylmethionine tRNA ribosyltransferase

Gene name queA
Synonyms
Essential no
Product S-adenosylmethionine tRNA ribosyltransferase
Function tRNA modification
Gene expression levels in SubtiExpress: queA
MW, pI 38 kDa, 5.047
Gene length, protein length 1026 bp, 342 aa
Immediate neighbours tgt, yrzS
Sequences Protein DNA Advanced_DNA
Genetic context
QueA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
QueA expression.png
























Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU27720

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: S-adenosylmethionine + 7-aminomethyl-7-deazaguanosine = methionine + adenine + epoxyqueuosine (according to Swiss-Prot)
  • Protein family: queA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Clemens Grimm, Ralf Ficner, Tanja Sgraja, Peter Haebel, Gerhard Klebe, Klaus Reuter
Crystal structure of Bacillus subtilis S-adenosylmethionine:tRNA ribosyltransferase-isomerase.
Biochem Biophys Res Commun: 2006, 351(3);695-701
[PubMed:17083917] [WorldCat.org] [DOI] (P p)

C Grimm, G Klebe, R Ficner, K Reuter
Screening orthologs as an important variable in crystallization: preliminary X-ray diffraction studies of the tRNA-modifying enzyme S-adenosyl-methionine:tRNA ribosyl transferase/isomerase.
Acta Crystallogr D Biol Crystallogr: 2000, 56(Pt 4);484-8
[PubMed:10739928] [WorldCat.org] [DOI] (P p)