Difference between revisions of "TopA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA replication]] | |style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA replication]] | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16120 topA] |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 78 kDa, 9.023 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 78 kDa, 9.023 | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dprA]]'', ''[[trmFO]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dprA]]'', ''[[trmFO]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16120 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16120 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16120 Advanced_DNA] |
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Revision as of 12:49, 13 May 2013
- Description: DNA topoisomerase I
| Gene name | topA |
| Synonyms | topI |
| Essential | no |
| Product | DNA topoisomerase I |
| Function | DNA replication |
| Gene expression levels in SubtiExpress: topA | |
| MW, pI | 78 kDa, 9.023 |
| Gene length, protein length | 2073 bp, 691 aa |
| Immediate neighbours | dprA, trmFO |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16120
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
- Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- TopA interacts with the RNA polymerase PubMed
Database entries
- Structure:
- UniProt: P39814
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: topA (according to DBTBS)
- Regulation:
- Regulatory mechanism:
- Additional information:
- translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
