Difference between revisions of "Rnc"

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|style="background:#ABCDEF;" align="center"|'''Function''' || cleaves both 5'- and 3'-sites <br/>of the small cytoplasmic RNA precursor (''[[scr]]'')
 
|style="background:#ABCDEF;" align="center"|'''Function''' || cleaves both 5'- and 3'-sites <br/>of the small cytoplasmic RNA precursor (''[[scr]]'')
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU15930 rnc]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15930 rnc]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acpA]]'', ''[[smc]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acpA]]'', ''[[smc]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13466&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15930 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:rnc_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:rnc_context.gif]]

Revision as of 12:49, 13 May 2013

  • Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria

Gene name rnc
Synonyms rncS
Essential yes
Product endoribonuclease III
Function cleaves both 5'- and 3'-sites
of the small cytoplasmic RNA precursor (scr)
Gene expression levels in SubtiExpress: rnc
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 28 kDa, 8.076
Gene length, protein length 747 bp, 249 aa
Immediate neighbours acpA, smc
Sequences Protein DNA Advanced_DNA
Genetic context
Rnc context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Rnc expression.png















Categories containing this gene/protein

Rnases, translation, protein secretion, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU15930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
    • cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
    • degradation of txpA mRNA-RatA RNA duplex (encoded on the Skin element) PubMed
    • degradation of yonT mRNA-as-yonT RNA duplex (encoded on the SP-beta prophage) PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number: 3.1.26.3

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

Your additional remarks

References

Reviews


Original publications

Durand S, Gilet L, Condon C  
The essential function of B. subtilis RNase III is to silence foreign toxin genes. 
PLoS Genet. 2012 8(12): e1003181.
PubMed:23300471