Difference between revisions of "PriA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA replication]] initiation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA replication]] initiation
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU15710 priA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15710 priA]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PriA PriA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PriA PriA]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yloI]]'', ''[[def]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yloI]]'', ''[[def]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13444&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15710 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15710 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15710 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:priA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:priA_context.gif]]

Revision as of 12:47, 13 May 2013

  • Description: primosomal replication factor Y, part of the replisome

Gene name priA
Synonyms yloJ
Essential yes PubMed
Product primosomal replication factor Y
Function DNA replication initiation
Gene expression levels in SubtiExpress: priA
Interactions involving this protein in SubtInteract: PriA
MW, pI 91 kDa, 8.279
Gene length, protein length 2415 bp, 805 aa
Immediate neighbours yloI, def
Sequences Protein DNA Advanced_DNA
Genetic context
PriA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PriA expression.png
























Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU15710

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408] [WorldCat.org] [DOI] (I p)

François Lecointe, Céline Sérèna, Marion Velten, Audrey Costes, Stephen McGovern, Jean-Christophe Meile, Jeffrey Errington, S Dusko Ehrlich, Philippe Noirot, Patrice Polard
Anticipating chromosomal replication fork arrest: SSB targets repair DNA helicases to active forks.
EMBO J: 2007, 26(19);4239-51
[PubMed:17853894] [WorldCat.org] [DOI] (P p)

S Marsin, S McGovern, S D Ehrlich, C Bruand, P Polard
Early steps of Bacillus subtilis primosome assembly.
J Biol Chem: 2001, 276(49);45818-25
[PubMed:11585815] [WorldCat.org] [DOI] (P p)

H Masai, J Deneke, Y Furui, T Tanaka, K I Arai
Escherichia coli and Bacillus subtilis PriA proteins essential for recombination-dependent DNA replication: involvement of ATPase/helicase activity of PriA for inducible stable DNA replication.
Biochimie: 1999, 81(8-9);847-57
[PubMed:10572298] [WorldCat.org] [DOI] (P p)