Difference between revisions of "Ogt"
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|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair | |style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13540 ogt] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[kinE]]'', ''[[mtnA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[kinE]]'', ''[[mtnA]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13540 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13540 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13540 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ogt_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ogt_context.gif]] | ||
Revision as of 12:39, 13 May 2013
- Description: O6-methylguanine DNA alkyltransferase
| Gene name | ogt |
| Synonyms | dat |
| Essential | no |
| Product | O6-methylguanine DNA alkyltransferase |
| Function | DNA repair |
| Gene expression levels in SubtiExpress: ogt | |
| Function and regulation of this protein in SubtiPathways: Phosphorelay | |
| MW, pI | 18 kDa, 7.092 |
| Gene length, protein length | 495 bp, 165 aa |
| Immediate neighbours | kinE, mtnA |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13540
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine (according to Swiss-Prot)
- Protein family: MGMT family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P11742
- KEGG entry: [3]
- E.C. number: 2.1.1.63
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
J A Rafferty, J E Wibley, P Speers, I Hickson, G P Margison, P C Moody, K T Douglas
The potential role of glycine-160 of human O6-alkylguanine-DNA alkyltransferase in reaction with O6-benzylguanine as determined by site-directed mutagenesis and molecular modelling comparisons.
Biochim Biophys Acta: 1997, 1342(1);90-102
[PubMed:9366274]
[WorldCat.org]
[DOI]
(P p)
F Morohoshi, N Munakata
Isolation of a Bacillus subtilis mutant defective in constitutive O6-alkylguanine-DNA alkyltransferase.
Mutat Res: 1990, 235(1);15-23
[PubMed:2105461]
[WorldCat.org]
[DOI]
(P p)
F Morohoshi, K Hayashi, N Munakata
Bacillus subtilis gene coding for constitutive O6-methylguanine-DNA alkyltransferase.
Nucleic Acids Res: 1989, 17(16);6531-43
[PubMed:2506524]
[WorldCat.org]
[DOI]
(P p)
