HtpX

• Description: stress-responsive membrane protease
  
  

Gene name	htpX
Synonyms	ykrL
Essential	no
Product	membrane protease
Function	quality control of membrane proteins
Gene expression levels in SubtiExpress: htpX
MW, pI	32 kDa, 10.157
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	ykrK, ktrD
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, proteolysis

This gene is a member of the following regulons

Rok regulon, YkrK regulon,

The gene

Basic information

• Locus tag: BSU13490

Phenotypes of a mutant

• increased sensitivity to membrane protein overproduction PubMed
• increased sensitivity to dissipation of the membrane potential by the addition of valinomycin PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase M48B family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31657

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: htpX PubMed

• Expression browser: htpX PubMed

• Sigma factor:

• Regulation:
  • induced by membrane protein overproduction (YkrK) PubMed
  • induced by dissipation of membrane potential (ΔΨ) by addition of a sublethal concentration of valinomycin PubMed
  • expression is increased due to mutations in resE, resB, sdhC, and sdhA PubMed
  • induced by salt stress PubMed

• Regulatory mechanism:
  • YkrK: transcription repression PubMed
  • Rok: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ta-Hui Lin, Shih-Chien Huang, Gwo-Chyuan Shaw
Reexamining transcriptional regulation of the Bacillus subtilis htpX gene and the ykrK gene, encoding a novel type of transcriptional regulator, and redefining the YkrK operator.
J Bacteriol: 2012, 194(24);6758-65
[PubMed:23042994] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Hein Trip, Patricia J van-der Veek, Oscar P Kuipers
Comparative transcriptional analysis of Bacillus subtilis cells overproducing either secreted proteins, lipoproteins or membrane proteins.
Microb Cell Fact: 2012, 11;66
[PubMed:22624725] [WorldCat.org] [DOI] (I e)

Bogumila C Marciniak, Hein Trip, Fabrizia Fusetti, Oscar P Kuipers
Regulation of ykrL (htpX) by Rok and YkrK, a novel type of regulator in Bacillus subtilis.
J Bacteriol: 2012, 194(11);2837-45
[PubMed:22447908] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)