IspA

• Description: intracellular serine protease
  
  

Gene name	ispA
Synonyms
Essential	no
Product	intracellular serine protease
Function	protein degradation
Gene expression levels in SubtiExpress: ispA
MW, pI	33 kDa, 4.522
Gene length, protein length	957 bp, 319 aa
Immediate neighbours	metE, rsbRB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nitrogen sources other than amino acids, proteolysis

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU13190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase S8 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P11018

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ispA (according to DBTBS)

• Expression browser: ispA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: