Difference between revisions of "FabF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU11340 fabF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11340 fabF]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabHA]]'', ''[[yjaZ]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabHA]]'', ''[[yjaZ]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12975&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11340 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11340 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11340 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:fabF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:fabF_context.gif]]

Revision as of 12:32, 13 May 2013

  • Description: beta-ketoacyl-acyl carrier protein synthase II, involved in the control of membrane fluidity

Gene name fabF
Synonyms yjaY
Essential yes PubMed
Product beta-ketoacyl-acyl carrier protein synthase II
Function fatty acid biosynthesis
Gene expression levels in SubtiExpress: fabF
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis
MW, pI 43 kDa, 4.768
Gene length, protein length 1239 bp, 413 aa
Immediate neighbours fabHA, yjaZ
Sequences Protein DNA Advanced_DNA
Genetic context
FabF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FabF expression.png




























Categories containing this gene/protein

membrane dynamics, biosynthesis of lipids, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

FapR regulon, SigW regulon

The gene

Basic information

  • Locus tag: BSU11340

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein] (according to Swiss-Prot)
    • SigW-dependent expression of fabF and the yuaF-floT-yuaI operon result in reduced membrane fluidity PubMed
  • Protein family: beta-ketoacyl-ACP synthases family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1OX0 (the protein from Streptococcus pneumoniae) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • fabHA: expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
    • fabHA: induced upon fatty acid biosynthesis inhibition PubMed
    • fabF: induced by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100 (SigW) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed

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