Difference between revisions of "PrkA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU08970 prkA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08970 prkA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 72 kDa, 5.459   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 72 kDa, 5.459   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhbF]]'', ''[[yhbH]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhbF]]'', ''[[yhbH]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12725&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU08970 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU08970 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU08970 Advanced_DNA]
 
|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:prkA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:prkA_context.gif]]

Revision as of 12:24, 13 May 2013

  • Description: putative serine protein kinase

Gene name prkA
Synonyms
Essential no
Product putative serine protein kinase
Function unknown
Gene expression levels in SubtiExpress: prkA
MW, pI 72 kDa, 5.459
Gene length, protein length 1893 bp, 631 aa
Immediate neighbours yhbF, yhbH
Sequences Protein DNA Advanced_DNA
Genetic context
PrkA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PrkA expression.png















Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU08970

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: prkA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jihen Tagourti, Ahmed Landoulsi, Gilbert Richarme
Cloning, expression, purification and characterization of the stress kinase YeaG from Escherichia coli.
Protein Expr Purif: 2008, 59(1);79-85
[PubMed:18276156] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

C Fischer, C Geourjon, C Bourson, J Deutscher
Cloning and characterization of the Bacillus subtilis prkA gene encoding a novel serine protein kinase.
Gene: 1996, 168(1);55-60
[PubMed:8626065] [WorldCat.org] [DOI] (P p)