Difference between revisions of "PcrB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown | |style="background:#ABCDEF;" align="center"|'''Function''' || unknown | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06600 pcrB] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 4.204 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 4.204 | ||
| Line 21: | Line 21: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yerD]]'', ''[[pcrA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yerD]]'', ''[[pcrA]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06600 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06600 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06600 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:pcrB_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:pcrB_context.gif]] | ||
Revision as of 12:17, 13 May 2013
- Description: heptaprenylglyceryl phosphate synthase
| Gene name | pcrB |
| Synonyms | yerE |
| Essential | no |
| Product | heptaprenylglyceryl phosphate synthase |
| Function | unknown |
| Gene expression levels in SubtiExpress: pcrB | |
| MW, pI | 24 kDa, 4.204 |
| Gene length, protein length | 684 bp, 228 aa |
| Immediate neighbours | yerD, pcrA |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06600
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- production of heptaprenylglyceryl phosphate from heptaprenyl diphosphate and glycerol-1-phosphate PubMed
- Protein family: GGGP synthase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- forms dimers PubMed
Database entries
- UniProt: O34790
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Feifei Ren, Xinxin Feng, Tzu-Ping Ko, Chun-Hsiang Huang, Yumei Hu, Hsiu-Chien Chan, Yi-Liang Liu, Ke Wang, Chun-Chi Chen, Xuefei Pang, Miao He, Yujie Li, Eric Oldfield, Rey-Ting Guo
Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus.
Chembiochem: 2013, 14(2);195-9
[PubMed:23322418]
[WorldCat.org]
[DOI]
(I p)
David Peterhoff, Hermann Zellner, Harald Guldan, Rainer Merkl, Reinhard Sterner, Patrick Babinger
Dimerization determines substrate specificity of a bacterial prenyltransferase.
Chembiochem: 2012, 13(9);1297-303
[PubMed:22614947]
[WorldCat.org]
[DOI]
(I p)
Harald Guldan, Frank-Michael Matysik, Marco Bocola, Reinhard Sterner, Patrick Babinger
Functional assignment of an enzyme that catalyzes the synthesis of an archaea-type ether lipid in bacteria.
Angew Chem Int Ed Engl: 2011, 50(35);8188-91
[PubMed:21761520]
[WorldCat.org]
[DOI]
(I p)
Harald Guldan, Reinhard Sterner, Patrick Babinger
Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis.
Biochemistry: 2008, 47(28);7376-84
[PubMed:18558723]
[WorldCat.org]
[DOI]
(I p)
M A Petit, E Dervyn, M Rose, K D Entian, S McGovern, S D Ehrlich, C Bruand
PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication.
Mol Microbiol: 1998, 29(1);261-73
[PubMed:9701819]
[WorldCat.org]
[DOI]
(P p)
