Difference between revisions of "YabT"

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|style="background:#ABCDEF;" align="center"|'''Function''' || control of DNA integrity during spore development
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of DNA integrity during spore development
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00660 yabT]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00660 yabT]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/YabT YabT]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/YabT YabT]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yabS]]'', ''[[tilS]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yabS]]'', ''[[tilS]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11842&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00660 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yabT_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yabT_context.gif]]

Revision as of 11:56, 13 May 2013

  • Description: Ser/Thr kinase, controls DNA integrity during spore development

Gene name yabT
Synonyms
Essential no
Product Ser/Thr kinase
Function control of DNA integrity during spore development
Gene expression levels in SubtiExpress: yabT
Interactions involving this protein in SubtInteract: YabT
MW, pI 37 kDa, 9.943
Gene length, protein length 1014 bp, 338 aa
Immediate neighbours yabS, tilS
Sequences Protein DNA Advanced_DNA
Genetic context
YabT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YabT expression.png















Categories containing this gene/protein

protein modification, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

  • Locus tag: BSU00660

Phenotypes of a mutant

  • increased sensitivity to DNA damage during spore development PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
    • phosphorylation of RecA on Ser-2 PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • autophosphorylation is stimulated by non-specific binding to DNA PubMed
  • Localization:
    • colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
    • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
    • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
  • lacZ fusion:
  • translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References