Difference between revisions of "MutTA"
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
** removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by [[RNases]] {{PubMed|21925382}} | ** removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by [[RNases]] {{PubMed|21925382}} | ||
| + | ** RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position {{PubMed|23610425,23610407}} | ||
* '''Protein family:''' [[Nudix hydrolase]] family (according to Swiss-Prot) | * '''Protein family:''' [[Nudix hydrolase]] family (according to Swiss-Prot) | ||
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=4JZV 4JZV] {{PubMed|23610407}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/O35013 O35013] | * '''UniProt:''' [http://www.uniprot.org/uniprot/O35013 O35013] | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ytkD_3134983_3135459_-1 mutTA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ytkD_3134983_3135459_-1 mutTA] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|14761999}}, [[SigF]] {{PubMed|14761999}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|14761999}}, [[SigF]] {{PubMed|14761999}} |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
== Reviews == | == Reviews == | ||
| − | + | <pubmed> 22568516</pubmed> | |
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== Original publications == | == Original publications == | ||
| − | <pubmed>16513759,16497325, 14761999, 19011023 9387221 15576788 21925382 </pubmed> | + | <pubmed>16513759,16497325, 14761999, 19011023 9387221 15576788 21925382 23610425 23610407 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 07:42, 25 April 2013
- Description: error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity
| Gene name | mutTA |
| Synonyms | ytkD, rppH |
| Essential | no |
| Product | 8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase |
| Function | DNA repair, RNA degradation |
| Gene expression levels in SubtiExpress: mutTA | |
| MW, pI | 18 kDa, 5.952 |
| Gene length, protein length | 474 bp, 158 aa |
| Immediate neighbours | ytlD, ytkC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA repair/ recombination, sporulation proteins, RNases
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU30630
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by RNases PubMed
- RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position PubMed
- Protein family: Nudix hydrolase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O35013
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Martin Lehnik-Habrink, Richard J Lewis, Ulrike Mäder, Jörg Stülke
RNA degradation in Bacillus subtilis: an interplay of essential endo- and exoribonucleases.
Mol Microbiol: 2012, 84(6);1005-17
[PubMed:22568516]
[WorldCat.org]
[DOI]
(I p)
Original publications
Ping-kun Hsieh, Jamie Richards, Quansheng Liu, Joel G Belasco
Specificity of RppH-dependent RNA degradation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2013, 110(22);8864-9
[PubMed:23610425]
[WorldCat.org]
[DOI]
(I p)
Jérémie Piton, Valéry Larue, Yann Thillier, Audrey Dorléans, Olivier Pellegrini, Inés Li de la Sierra-Gallay, Jean-Jacques Vasseur, Françoise Debart, Carine Tisné, Ciarán Condon
Bacillus subtilis RNA deprotection enzyme RppH recognizes guanosine in the second position of its substrates.
Proc Natl Acad Sci U S A: 2013, 110(22);8858-63
[PubMed:23610407]
[WorldCat.org]
[DOI]
(I p)
Jamie Richards, Quansheng Liu, Olivier Pellegrini, Helena Celesnik, Shiyi Yao, David H Bechhofer, Ciarán Condon, Joel G Belasco
An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.
Mol Cell: 2011, 43(6);940-9
[PubMed:21925382]
[WorldCat.org]
[DOI]
(I p)
Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023]
[WorldCat.org]
[DOI]
(I p)
Francisco X Castellanos-Juárez, Carlos Alvarez-Alvarez, Ronald E Yasbin, Barbara Setlow, Peter Setlow, Mario Pedraza-Reyes
YtkD and MutT protect vegetative cells but not spores of Bacillus subtilis from oxidative stress.
J Bacteriol: 2006, 188(6);2285-9
[PubMed:16513759]
[WorldCat.org]
[DOI]
(P p)
Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325]
[WorldCat.org]
[DOI]
(P p)
Wenlian Xu, Candice R Jones, Christopher A Dunn, Maurice J Bessman
Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily.
J Bacteriol: 2004, 186(24);8380-4
[PubMed:15576788]
[WorldCat.org]
[DOI]
(P p)
Martha I Ramírez, Francisco X Castellanos-Juárez, Ronald E Yasbin, Mario Pedraza-Reyes
The ytkD (mutTA) gene of Bacillus subtilis encodes a functional antimutator 8-Oxo-(dGTP/GTP)ase and is under dual control of sigma A and sigma F RNA polymerases.
J Bacteriol: 2004, 186(4);1050-9
[PubMed:14761999]
[WorldCat.org]
[DOI]
(P p)
Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221]
[WorldCat.org]
[DOI]
(P p)
