CwlJ

• Description: cell wall hydrolase
  
  

Gene name	cwlJ
Synonyms	ycbQ
Essential	no
Product	cell wall hydrolase
Function	spore germination
Gene expression levels in SubtiExpress: cwlJ
MW, pI	16 kDa, 9.354
Gene length, protein length	426 bp, 142 aa
Immediate neighbours	ycbP, ycbR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, sporulation proteins, germination

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU02600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cwlJ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer edge of the spore cortex PubMed

Database entries

• Structure:

• UniProt: P42249

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cwlJ PubMed

• Expression browser: cwlJ PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1G19 ( cwlJ::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yunfeng Li, Xuan Y Butzin, Andrew Davis, Barbara Setlow, George Korza, Fatma Işik Üstok, Graham Christie, Peter Setlow, Bing Hao
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
J Bacteriol: 2013, 195(11);2530-40
[PubMed:23543708] [WorldCat.org] [DOI] (I p)

Xuan Yi, Peter Setlow
Studies of the commitment step in the germination of spores of bacillus species.
J Bacteriol: 2010, 192(13);3424-33
[PubMed:20435722] [WorldCat.org] [DOI] (I p)

Alicia Monroe, Peter Setlow
Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ.
J Bacteriol: 2006, 188(21);7609-16
[PubMed:16936016] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Katerina Ragkousi, Peter Setlow
Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores.
J Bacteriol: 2004, 186(17);5567-75
[PubMed:15317760] [WorldCat.org] [DOI] (P p)

Haridasan Chirakkal, Michele O'Rourke, Abdelmadjid Atrih, Simon J Foster, Anne Moir
Analysis of spore cortex lytic enzymes and related proteins in Bacillus subtilis endospore germination.
Microbiology (Reading): 2002, 148(Pt 8);2383-2392
[PubMed:12177332] [WorldCat.org] [DOI] (P p)

Irina Bagyan, Peter Setlow
Localization of the cortex lytic enzyme CwlJ in spores of Bacillus subtilis.
J Bacteriol: 2002, 184(4);1219-24
[PubMed:11807087] [WorldCat.org] [DOI] (P p)

M Paidhungat, K Ragkousi, P Setlow
Genetic requirements for induction of germination of spores of Bacillus subtilis by Ca(2+)-dipicolinate.
J Bacteriol: 2001, 183(16);4886-93
[PubMed:11466292] [WorldCat.org] [DOI] (P p)

S Ishikawa, K Yamane, J Sekiguchi
Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores.
J Bacteriol: 1998, 180(6);1375-80
[PubMed:9515903] [WorldCat.org] [DOI] (P p)