GerD

• Description: required for clustering of germinant receptors in the spore inner membrane
  
  

Gene name	gerD
Synonyms
Essential	no
Product	lipoprotein
Function	germination
Gene expression levels in SubtiExpress: gerD
Interactions involving this protein in SubtInteract: GerD
MW, pI	20 kDa, 4.862
Gene length, protein length	555 bp, 185 aa
Immediate neighbours	salA, kbaA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU01550

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: required for clustering of germinant receptors in the spore inner membrane PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • has an N-terminal signal sequence and a signal for fixation to lipid PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerD is part of the germinosome in the spore inner membrane PubMed
  • GerD-Lgt to fix GerD to diacylglycerol in the membrane PubMed

• Localization:
  • outer surface of the inner spore membrane PubMed
  • inner spore membrane, spore coat, cortex, germ cell wall, some GerD is also present in the soluble fraction PubMed

Database entries

• Structure:

• UniProt: P16450

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gerD PubMed

• Expression browser: gerD PubMed

• Sigma factor: SigF PubMed, SigG PubMed

• Regulation:
  • expression level depends on sporulation conditions (medim composition, temperature) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A677 ( gerD::erm), PubMed, available at BGSC
  • 1G17 ( gerD::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Anne Moir lab

Labs working on this gene/protein

Anne Moir, University of Sheffield, UK

References

Additional references: PubMed