SpoVAD

• Description: essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2+) into developing spores, likely germination protein
  
  

Gene name	spoVAD
Synonyms
Essential	no
Product	binding protein for DPA
Function	likely germination protein
Gene expression levels in SubtiExpress: spoVAD
Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr
MW, pI	35 kDa, 5.243
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	spoVAEB, spoVAC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU23410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • maybe involved in spore germination PubMed
  • binding of both DPA(2,6) and Ca(2+)-DPA(2,6) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer surface of the inner spore membrane PubMed

Database entries

• Structure: 3LM6

• UniProt: P40869

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA PubMed

• Expression browser: spoVAD PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Peter Setlow, University of Connecticut Health Center, USA

References

Additional publications: PubMed