Difference between revisions of "BslA"

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(Biological materials)
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* '''Mutant:'''
 
* '''Mutant:'''
** [[B. subtilis]] W939 [[yuaB]]::Cm (ATCC6051 based) {{PubMed|17850253}}
+
** ''B. subtilis'' W939 ''yuaB''::Cm (ATCC6051 based) {{PubMed|17850253}}
** [[B. subtilis]] NRS2097 [[yuaB]]::Cm (NCIB3610 based) {{PubMed|18978066}}
+
** ''B. subtilis'' NRS2097 ''yuaB''::Cm (NCIB3610 based) {{PubMed|18978066}}
** [[B. subtilis]] [[yuaB]]::Cm (168 based) {{PubMed|21097620}}
+
** ''B. subtilis'' ''yuaB''::Cm (168 based) {{PubMed|21097620}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
** pNW500 P[[yuaB-lacZ]] fusion in pDG1663 {{PubMed|18978066}}
+
** pNW500 P''yuaB-lacZ'' fusion in pDG1663 {{PubMed|18978066}}
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
** P[[yuaB-gfp]] fusion in pSG1151 vector: pSGyuaB {{PubMed|21097620}}
+
** P''yuaB-gfp'' fusion in pSG1151 vector: pSGyuaB {{PubMed|21097620}}
  
 
* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  

Revision as of 10:37, 5 January 2013

  • Description: amphiphilic protein, forms water-repellent surface layer of the biofilm

Gene name bslA
Synonyms yuaB
Essential no
Product biofilm surface layer
Function biofilm formation
MW, pI 19 kDa, 9.987
Gene length, protein length 543 bp, 181 aa
Immediate neighbours gbsR, ktrA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YuaB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YuaB expression.png
























Categories containing this gene/protein

biofilm formation

This gene is a member of the following regulons

AbrB regulon, DegU regulon

The gene

Basic information

  • Locus tag: BSU31080

Phenotypes of a mutant

  • reduced colony complexity PubMed
  • loss of surface repellency of the biofilm PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YweA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:
    • extracellular (signal peptide) PubMed
    • biofilm matrix in an extracellular polysaccharide-dependent manner PubMed
    • forms a layer on the biofilm surface PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • expression is down-regulated in a rok mutant (indirect effect) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • B. subtilis W939 yuaB::Cm (ATCC6051 based) PubMed
    • B. subtilis NRS2097 yuaB::Cm (NCIB3610 based) PubMed
    • B. subtilis yuaB::Cm (168 based) PubMed
  • Expression vector:
    • Physpank-yuaB based on vector pDR111: pDRyuaB2 PubMed
  • lacZ fusion:
    • pNW500 PyuaB-lacZ fusion in pDG1663 PubMed
  • GFP fusion:
    • PyuaB-gfp fusion in pSG1151 vector: pSGyuaB PubMed
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Akos T Kovács, Jordi van Gestel, Oscar P Kuipers
The protective layer of biofilm: a repellent function for a new class of amphiphilic proteins.
Mol Microbiol: 2012, 85(1);8-11
[PubMed:22607588] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed