Difference between revisions of "SkfB"
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| − | * '''Description:''' | + | * '''Description:''' thioether bond forming radical SAM enzyme, catalyzes the first step in the maturation of spore killing factor <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || thioether bond forming radical SAM enzyme |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || maturation of spore killing factor |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01920 skfB] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01920 skfB] | ||
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SkfB SkfB] | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 4.934 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 4.934 | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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| − | |||
| − | |||
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<br/><br/> | <br/><br/> | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| + | ** generates a thioether bond in [[SkfA]], which links the cysteine residue Cys4 with the α-carbon of the methionine residue Met12 {{PubMed|23282011}} | ||
* '''Protein family:''' moaA/nifB/pqqE family (according to Swiss-Prot) | * '''Protein family:''' moaA/nifB/pqqE family (according to Swiss-Prot) | ||
| Line 86: | Line 85: | ||
* '''Modification:''' | * '''Modification:''' | ||
| − | * '''Cofactor(s):''' contains | + | * '''Cofactor(s):''' contains two [4Fe-4S] clusters {{PubMed|23282011}} |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[SkfA]]-[[SkfB]] {{PubMed|23282011}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| Line 148: | Line 148: | ||
<pubmed>20955377 </pubmed> | <pubmed>20955377 </pubmed> | ||
==Original Publications== | ==Original Publications== | ||
| − | <pubmed>12817086,15812018,,16816204,14651647,15687200,15687200,17720793, ,17449691,18840696 </pubmed> | + | <pubmed>12817086,15812018,23282011,16816204,14651647,15687200,15687200,17720793, ,17449691,18840696 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:23, 4 January 2013
- Description: thioether bond forming radical SAM enzyme, catalyzes the first step in the maturation of spore killing factor
| Gene name | skfB |
| Synonyms | ybcP, ybcQ |
| Essential | no |
| Product | thioether bond forming radical SAM enzyme |
| Function | maturation of spore killing factor |
| Gene expression levels in SubtiExpress: skfB | |
| Interactions involving this protein in SubtInteract: SkfB | |
| MW, pI | 45 kDa, 4.934 |
| Gene length, protein length | 1230 bp, 410 aa |
| Immediate neighbours | skfA, skfC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
toxins, antitoxins and immunity against toxins
This gene is a member of the following regulons
AbrB regulon, PhoP regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU01920
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: moaA/nifB/pqqE family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): contains two [4Fe-4S] clusters PubMed
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O31423
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information: Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript. The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
José Eduardo González-Pastor
Cannibalism: a social behavior in sporulating Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(3);415-24
[PubMed:20955377]
[WorldCat.org]
[DOI]
(I p)
Original Publications
Leif Flühe, Olaf Burghaus, Beata M Wieckowski, Tobias W Giessen, Uwe Linne, Mohamed A Marahiel
Two [4Fe-4S] clusters containing radical SAM enzyme SkfB catalyze thioether bond formation during the maturation of the sporulation killing factor.
J Am Chem Soc: 2013, 135(3);959-62
[PubMed:23282011]
[WorldCat.org]
[DOI]
(I p)
Allison V Banse, Arnaud Chastanet, Lilah Rahn-Lee, Errett C Hobbs, Richard Losick
Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2008, 105(40);15547-52
[PubMed:18840696]
[WorldCat.org]
[DOI]
(I p)
Mark A Strauch, Benjamin G Bobay, John Cavanagh, Fude Yao, Angelo Wilson, Yoann Le Breton
Abh and AbrB control of Bacillus subtilis antimicrobial gene expression.
J Bacteriol: 2007, 189(21);7720-32
[PubMed:17720793]
[WorldCat.org]
[DOI]
(P p)
Akihito Ochiai, Takafumi Itoh, Akiko Kawamata, Wataru Hashimoto, Kousaku Murata
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl Environ Microbiol: 2007, 73(12);3803-13
[PubMed:17449691]
[WorldCat.org]
[DOI]
(P p)
Nicholas E E Allenby, Carys A Watts, Georg Homuth, Zoltán Prágai, Anil Wipat, Alan C Ward, Colin R Harwood
Phosphate starvation induces the sporulation killing factor of Bacillus subtilis.
J Bacteriol: 2006, 188(14);5299-303
[PubMed:16816204]
[WorldCat.org]
[DOI]
(P p)
Helga Westers, Peter G Braun, Lidia Westers, Haike Antelmann, Michael Hecker, Jan D H Jongbloed, Hirofumi Yoshikawa, Teruo Tanaka, Jan Maarten van Dijl, Wim J Quax
Genes involved in SkfA killing factor production protect a Bacillus subtilis lipase against proteolysis.
Appl Environ Microbiol: 2005, 71(4);1899-908
[PubMed:15812018]
[WorldCat.org]
[DOI]
(P p)
Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
José E González-Pastor, Errett C Hobbs, Richard Losick
Cannibalism by sporulating bacteria.
Science: 2003, 301(5632);510-3
[PubMed:12817086]
[WorldCat.org]
[DOI]
(I p)
