YtsJ

• Description: malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH
  
  

Gene name	ytsJ
Synonyms
Essential	no
Product	NADP-dependent malate dehydrogenase
Function	malate utilization
Gene expression levels in SubtiExpress: ytsJ
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	43 kDa, 5.046
Gene length, protein length	1230 bp, 410 aa
Immediate neighbours	accD, dnaE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

The gene

Basic information

• Locus tag: BSU29220

Phenotypes of a mutant

Poor growth with malate as single carbon source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate + NADP⁺ = pyruvate + CO₂ + NADPH (according to Swiss-Prot) malate <--> pyruvate

• Protein family: malic enzymes family (according to Swiss-Prot)

• Paralogous protein(s): MleA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed cytoplasm protein PubMed

Database entries

• Structure: 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)

• UniProt: O34962

• KEGG entry: [3]

• E.C. number: 1.1.1.38

Expression and regulation

• Operon: ytsJ PubMed

• Expression browser: ytsJ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP612 (spc), available in Jörg Stülke's lab
  • GP1143 (spc), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• FLAG-tag construct: GP1131 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

Martin Rühl, Dominique Le Coq, Stéphane Aymerich, Uwe Sauer
13C-flux analysis reveals NADPH-balancing transhydrogenation cycles in stationary phase of nitrogen-starving Bacillus subtilis.
J Biol Chem: 2012, 287(33);27959-70
[PubMed:22740702] [WorldCat.org] [DOI] (I p)