Difference between revisions of "EngA"

From SubtiWiki
Jump to: navigation, search
m (moved YphC to EngA over redirect: 23056455)
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || ribosome assembly  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || ribosome assembly  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22840 yphC]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22840 engA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.248   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.248   
Line 35: Line 35:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
 
 
 
<br/><br/>
 
<br/><br/>
  
Line 78: Line 74:
 
* '''Protein family:''' EngA subfamily (according to Swiss-Prot) Era/Obg family
 
* '''Protein family:''' EngA subfamily (according to Swiss-Prot) Era/Obg family
  
* '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YphC]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]
+
* '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 112: Line 108:
 
* '''Operon:'''  
 
* '''Operon:'''  
  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 yphC] {{PubMed|22383849}}
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 engA] {{PubMed|22383849}}
  
 
* '''Sigma factor:'''  
 
* '''Sigma factor:'''  
Line 127: Line 123:
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP846,  available in [[Stülke]] lab
+
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP846,  available in [[Jörg Stülke]]'s lab
  
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
Line 148: Line 144:
  
 
==Original publications==
 
==Original publications==
<pubmed>12427945, 16997968,16894162 16682769 </pubmed>
+
<pubmed>12427945, 16997968,16894162 16682769 23056455</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:57, 14 October 2012

  • Description: GTPase essential for ribosome 50S subunit assembly

Gene name yphC
Synonyms engA
Essential yes PubMed
Product GTPase
Function ribosome assembly
Gene expression levels in SubtiExpress: engA
MW, pI 48 kDa, 5.248
Gene length, protein length 1308 bp, 436 aa
Immediate neighbours gpsA, seaA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YphC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YphC expression.png















Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22840

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: EngA subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2HJG (complex with GDP)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP846, available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews


Original publications