MgtE

• Description: magnesium transport via permease (no H+)
  
  

Gene name	mgtE
Synonyms	ykoK
Essential	no
Product	magnesium transport via permease (no H+)
Function	magnesium uptake
Gene expression levels in SubtiExpress: mgtE
MW, pI	50 kDa, 4.609
Gene length, protein length	1353 bp, 451 aa
Immediate neighbours	ykzD, tnrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), membrane proteins

This gene is a member of the following regulons

M-box

The gene

Basic information

• Locus tag: BSU13300

Database entries

• DBTBS entry: no entry
• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SLC41A transporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 2QBZ (the mgtE riboswitch)

• UniProt: O34442

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: mgtE PubMed

• Sigma factor:

• Regulation: induced at low magnesium concentration (M-box) PubMed

• Regulatory mechanism: M-box: Mg(2+)-responsive riboswitch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jaya Sahni, Yumei Song, Andrew M Scharenberg
The B. subtilis MgtE magnesium transporter can functionally compensate TRPM7-deficiency in vertebrate B-cells.
PLoS One: 2012, 7(9);e44452
[PubMed:22970223] [WorldCat.org] [DOI] (I p)

Arati Ramesh, Catherine A Wakeman, Wade C Winkler
Insights into metalloregulation by M-box riboswitch RNAs via structural analysis of manganese-bound complexes.
J Mol Biol: 2011, 407(4);556-70
[PubMed:21315082] [WorldCat.org] [DOI] (I p)

Charles E Dann, Catherine A Wakeman, Cecelia L Sieling, Stephanie C Baker, Irnov Irnov, Wade C Winkler
Structure and mechanism of a metal-sensing regulatory RNA.
Cell: 2007, 130(5);878-92
[PubMed:17803910] [WorldCat.org] [DOI] (P p)

Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624] [WorldCat.org] [DOI] (P p)