LytD

• Description: glucosaminidase
  
  

Gene name	lytD
Synonyms	cwlG
Essential	no
Product	glucosaminidase
Function	major autolysin, cell separation
Gene expression levels in SubtiExpress: lytD
MW, pI	95 kDa, 9.935
Gene length, protein length	2640 bp, 880 aa
Immediate neighbours	tagC, pmi
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU35780

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relexed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)₂)Asn-structure (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 73 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P39848

• KEGG entry: [3]

• E.C. number: 3.2.1.96

Expression and regulation

• Operon: lytD PubMed

• Expression browser: lytD PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A788 ( lytD::tet), PubMed, available at BGSC
  • 1A792 ( lytD::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: