Difference between revisions of "WprA"
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* '''Mutant:''' | * '''Mutant:''' | ||
+ | ** 1A1021 ( ''wprA''::''kan''), {{PubMed|20400548}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1021&Search=1A1021 BGSC] | ||
* '''Expression vector:''' | * '''Expression vector:''' |
Revision as of 12:15, 19 September 2012
- Description: secreted quality control protease
Gene name | wprA |
Synonyms | yisM |
Essential | no |
Product | secreted quality control protease |
Function | protein quality control |
Gene expression levels in SubtiExpress: wprA | |
MW, pI | 96 kDa, 9.58 |
Gene length, protein length | 2682 bp, 894 aa |
Immediate neighbours | yisL, yisN |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
cell wall/ other, proteolysis,
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10770
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: peptidase S8 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed, membrane PubMed
Database entries
- Structure:
- UniProt: P54423
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
- An antisense RNA is predicted forwprA PubMed
- the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Laxmi Krishnappa, Carmine G Monteferrante, Jan Maarten van Dijl
Degradation of the twin-arginine translocation substrate YwbN by extracytoplasmic proteases of Bacillus subtilis.
Appl Environ Microbiol: 2012, 78(21);7801-4
[PubMed:22923395]
[WorldCat.org]
[DOI]
(I p)
Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538]
[WorldCat.org]
[DOI]
(I e)
Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. Mol Microbiol. 2011 81(6): 1459-1473. PubMed:21815947