MetN

• Description: methionine ABC transporter (ATP-binding protein)
  
  

Gene name	metN
Synonyms	yusC
Essential	no
Product	methionine ABC transporter (ATP-binding protein)
Function	methionine uptake
Gene expression levels in SubtiExpress: metN
Interactions involving this protein in SubtInteract: MetN
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	37 kDa, 7.303
Gene length, protein length	1023 bp, 341 aa
Immediate neighbours	metP, yusD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, S-box

The gene

Basic information

• Locus tag: BSU32750

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MetN-MetP-MetQ PubMed

• Localization: associated to the cell membrane, via MetP PubMed

Database entries

• Structure:

• UniProt: O32169

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: metN-metP-metQ PubMed

• Expression browser: metN PubMed

• Sigma factor:

• Regulation:
  • activated during growth in the presence of branched chain amino acids (CodY) PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism:
  • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
  • CodY: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A797 ( metN::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: