Difference between revisions of "DacB"

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* '''Mutant:'''
 
* '''Mutant:'''
 
** 1A744 ( ''dacB''::''cat''), {{PubMed|1644769}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A744&Search=1A744 BGSC]
 
** 1A744 ( ''dacB''::''cat''), {{PubMed|1644769}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A744&Search=1A744 BGSC]
 +
** 1A745 ( ''dacB''::''cat''), {{PubMed|1644769}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A745&Search=1A745 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 09:04, 19 September 2012

Gene name dacB
Synonyms
Essential no
Product penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
Function carboxypeptidase
Gene expression levels in SubtiExpress: dacB
MW, pI 42 kDa, 9.725
Gene length, protein length 1146 bp, 382 aa
Immediate neighbours spmA, ypuI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DacB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DacB expression.png
























Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU23190

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: modifies degree of cross-linking of glycan strands in peptidoglycan
  • Protein family: peptidase S11 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3MFD (beta-lactamase superfamily domain)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed