Difference between revisions of "WprA"
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| − | {{SubtiWiki regulon|[[YvrHb regulon]]}} | + | {{SubtiWiki regulon|[[YvrHb regulon]]}}, |
| + | {{SubtiWiki regulon|[[CcpA regulon]]}} | ||
=The gene= | =The gene= | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=wprA_1153789_1156473_1 wprA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=wprA_1153789_1156473_1 wprA] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''Sigma factor:''' [[SigA]] {{PubMed|22900538}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** repressed by glucose ([[CcpA]]) {{PubMed|22900538}} | ||
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' |
| + | ** [[CcpA]]: transcription repression {{PubMed|22900538}} | ||
| + | ** [[YvrHb]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
| + | <pubmed> 22900538</pubmed> | ||
<big>''Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J'' </big> | <big>''Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J'' </big> | ||
<big>'''RNA processing in ''Bacillus subtilis'': identification of targets of the essential RNase Y.''' </big> | <big>'''RNA processing in ''Bacillus subtilis'': identification of targets of the essential RNase Y.''' </big> | ||
Revision as of 16:49, 23 August 2012
- Description: cell wall-associated protein precursor
| Gene name | wprA |
| Synonyms | yisM |
| Essential | no |
| Product | cell wall-associated protein precursor |
| Function | unknown |
| Gene expression levels in SubtiExpress: wprA | |
| MW, pI | 96 kDa, 9.58 |
| Gene length, protein length | 2682 bp, 894 aa |
| Immediate neighbours | yisL, yisN |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10770
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: peptidase S8 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed, membrane PubMed
Database entries
- Structure:
- UniProt: P54423
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
- An antisense RNA is predicted forwprA PubMed
- the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538]
[WorldCat.org]
[DOI]
(I e)
Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. Mol Microbiol. 2011 81(6): 1459-1473. PubMed:21815947
