LigA

• Description: DNA ligase (NAD-dependent)
  
  

Gene name	ligA
Synonyms	lig, yerG
Essential	yes PubMed
Product	DNA ligase (NAD-dependent)
Function	replication
Gene expression levels in SubtiExpress: ligA
MW, pI	74 kDa, 4.708
Gene length, protein length	2004 bp, 668 aa
Immediate neighbours	pcrA, yerH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU06620

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: NAD⁺ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m) (according to Swiss-Prot)

• Protein family: BRCT domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1B04 (adenylation domain, Geobacillus stearothermophilus)

• UniProt: O31498

• KEGG entry: [3]

• E.C. number: 6.5.1.2

Expression and regulation

• Operon: pcrB-pcrA-ligA-yerH PubMed

• Expression browser: ligA PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: