HxlA

• Description: 3-hexulose-6-phosphate synthase
  
  

Gene name	hxlA
Synonyms	yckG
Essential	no
Product	3-hexulose-6-phosphate synthase
Function	ribulose monophosphate pathway for formaldehyde fixation
Gene expression levels in SubtiExpress: hxlA
MW, pI	22 kDa, 4.564
Gene length, protein length	630 bp, 210 aa
Immediate neighbours	hxlB, hxlR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

resistance against oxidative and electrophile stress

This gene is a member of the following regulons

HxlR regulon

The gene

Basic information

• Locus tag: BSU03460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde (according to Swiss-Prot)

• Protein family: HPS subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P42405

• KEGG entry: [3]

• E.C. number: 4.1.2.43

Expression and regulation

• Operon: hxlA-hxlB PubMed

• Expression browser: hxlA PubMed

• Sigma factor:

• Regulation: induction by formaldehyde (HxlR) PubMed

• Regulatory mechanism: transcription activation by HxlR PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Thi Thu Huyen Nguyen, Warawan Eiamphungporn, Ulrike Mäder, Manuel Liebeke, Michael Lalk, Michael Hecker, John D Helmann, Haike Antelmann
Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR).
Mol Microbiol: 2009, 71(4);876-94
[PubMed:19170879] [WorldCat.org] [DOI] (I p)

Nobuo Kato, Hiroya Yurimoto, Rudolf K Thauer
The physiological role of the ribulose monophosphate pathway in bacteria and archaea.
Biosci Biotechnol Biochem: 2006, 70(1);10-21
[PubMed:16428816] [WorldCat.org] [DOI] (P p)

H Yasueda, Y Kawahara, S Sugimoto
Bacillus subtilis yckG and yckF encode two key enzymes of the ribulose monophosphate pathway used by methylotrophs, and yckH is required for their expression.
J Bacteriol: 1999, 181(23);7154-60
[PubMed:10572115] [WorldCat.org] [DOI] (P p)