YabA

• Description: tethers DnaA to the polymerase-clamp protein DnaN
  
  

Gene name	yabA
Synonyms
Essential	no
Product	negative regulator of replication initiation
Function	positioning of DnaA
Gene expression levels in SubtiExpress: yabA
Interactions involving this protein in SubtInteract: YabA
MW, pI	13 kDa, 6.065
Gene length, protein length	357 bp, 119 aa
Immediate neighbours	yaaT, yabB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA replication

The gene

Basic information

• Locus tag: BSU00330

Phenotypes of a mutant

strongly reduced growth rate in minimal medium PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • negative control of replication initiation PubMed
  • associates with the oriC region in a DnaA-dependent manner, this limits the amount of DnaA that can bind the oriC region PubMed
  • YabA inhibits co-operative DNA binding by DnaA PubMed

• Protein family: yabA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: leucine zipper

• Modification:

• Cofactor(s): Zn cluster

• Effectors of protein activity:
  • DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed

• Interactions: DnaA-YabA-DnaN PubMed

• Localization:

Database entries

• Structure:

• UniProt: P37542

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yabA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Philippe Noirot, Jouy-en-Josas, France Homepage
Alan Grossman, Cambridge, MA, USA Homepage

References

Reviews

Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337] [WorldCat.org] [DOI] (I p)