YuaF

• Description: NfeD2, role in maintaining membrane integrity during conditions of cellular stress, confers (together with FloT) resistance to cefuroxime
  
  

Gene name	yuaF
Synonyms
Essential	no
Product	NfeD family protein NfeD2
Function	involved in the control of membrane fluidity
Gene expression levels in SubtiExpress: yuaF
Interactions involving this protein in SubtInteract: YuaF
MW, pI	18 kDa, 4.042
Gene length, protein length	522 bp, 174 aa
Immediate neighbours	floT, yuaE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane dynamics, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

• Locus tag: BSU31020

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • SigW-dependent expression of fabF and the yuaF-floT-yuaI operon result in reduced membrane fluidity PubMed

• Protein family: NfeD family (with YqeZ)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YuaF-FloT PubMed

• Localization:
  • cell membrane, in focal structures PubMed

Database entries

• Structure: 2K14

• UniProt: O32077

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yuaF-floT-yuaI PubMed

• Expression browser: yuaF PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • expressed upon cell wall stress (SigW) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Felix Dempwolff, Heiko M Möller, Peter L Graumann
Synthetic motility and cell shape defects associated with deletions of flotillin/reggie paralogs in Bacillus subtilis and interplay of these proteins with NfeD proteins.
J Bacteriol: 2012, 194(17);4652-61
[PubMed:22753055] [WorldCat.org] [DOI] (I p)

Christina A Walker, Markus Hinderhofer, David J Witte, Winfried Boos, Heiko M Möller
Solution structure of the soluble domain of the NfeD protein YuaF from Bacillus subtilis.
J Biomol NMR: 2008, 42(1);69-76
[PubMed:18696230] [WorldCat.org] [DOI] (P p)

X Huang, A Gaballa, M Cao, J D Helmann
Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W.
Mol Microbiol: 1999, 31(1);361-71
[PubMed:9987136] [WorldCat.org] [DOI] (P p)