CwlA

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	cwlA
Synonyms
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	minor autolysin
Gene expression levels in SubtiExpress: cwlA
MW, pI	29 kDa, 10.4
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	yqxI, yqxH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, Skin element

The gene

Basic information

• Locus tag: BSU25900

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_2 domain (like BlyA, CwlH, XlyA, XlyB)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P24808

• KEGG entry: [2]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon:

• Expression browser: cwlA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

A Kuroda, M Imazeki, J Sekiguchi
Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis.
FEMS Microbiol Lett: 1991, 65(1);9-13
[PubMed:1908396] [WorldCat.org] [DOI] (P p)