Difference between revisions of "NasE"
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| − | + | * '''Description:''' assimilatory nitrite reductase (subunit) <br/><br/> | |
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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of nitrite as nitrogen source | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of nitrite as nitrogen source | ||
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NasE NasE] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NasE NasE] | ||
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|colspan="2" | '''Genetic context''' <br/> [[Image:nasE_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:nasE_context.gif]] | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
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__TOC__ | __TOC__ | ||
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* '''Operon:''' ''[[nasB]]-[[nasC]]-[[nasD]]-[[nasE]]-[[nasF]]'' {{PubMed|7836289}} | * '''Operon:''' ''[[nasB]]-[[nasC]]-[[nasD]]-[[nasE]]-[[nasF]]'' {{PubMed|7836289}} | ||
| − | * | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7836289}} |
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* '''Regulation:''' | * '''Regulation:''' | ||
Revision as of 11:05, 7 August 2012
- Description: assimilatory nitrite reductase (subunit)
| Gene name | nasE |
| Synonyms | nasBD, nirD |
| Essential | no |
| Product | assimilatory nitrite reductase (subunit) |
| Function | utilization of nitrite as nitrogen source |
| Interactions involving this protein in SubtInteract: NasE | |
| Metabolic function and regulation of this protein in SubtiPathways: Alternative nitrogen sources | |
| MW, pI | 11 kDa, 4.805 |
| Gene length, protein length | 318 bp, 106 aa |
| Immediate neighbours | nasF, nasD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
respiration, utilization of nitrogen sources other than amino acids
This gene is a member of the following regulons
Fur regulon, NsrR regulon, ResD regulon, TnrA regulon
The gene
Basic information
- Locus tag: BSU03290
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P42436
- KEGG entry: [3]
- E.C. number: 1.7.1.4
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Víctor M Luque-Almagro, Andrew J Gates, Conrado Moreno-Vivián, Stuart J Ferguson, David J Richardson, M Dolores Roldán
Bacterial nitrate assimilation: gene distribution and regulation.
Biochem Soc Trans: 2011, 39(6);1838-43
[PubMed:22103536]
[WorldCat.org]
[DOI]
(I p)
Original publications
Sushma Kommineni, Erik Yukl, Takahiro Hayashi, Jacob Delepine, Hao Geng, Pierre Moënne-Loccoz, Michiko M Nakano
Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter.
Mol Microbiol: 2010, 78(5);1280-93
[PubMed:21091510]
[WorldCat.org]
[DOI]
(I p)
Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456]
[WorldCat.org]
[DOI]
(P p)
Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818]
[WorldCat.org]
[DOI]
(P p)
M M Nakano, Y Zhu, M Lacelle, X Zhang, F M Hulett
Interaction of ResD with regulatory regions of anaerobically induced genes in Bacillus subtilis.
Mol Microbiol: 2000, 37(5);1198-207
[PubMed:10972836]
[WorldCat.org]
[DOI]
(P p)
M M Nakano, T Hoffmann, Y Zhu, D Jahn
Nitrogen and oxygen regulation of Bacillus subtilis nasDEF encoding NADH-dependent nitrite reductase by TnrA and ResDE.
J Bacteriol: 1998, 180(20);5344-50
[PubMed:9765565]
[WorldCat.org]
[DOI]
(P p)
L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114]
[WorldCat.org]
[DOI]
(P p)
K Ogawa, E Akagawa, K Yamane, Z W Sun, M LaCelle, P Zuber, M M Nakano
The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.
J Bacteriol: 1995, 177(5);1409-13
[PubMed:7868621]
[WorldCat.org]
[DOI]
(P p)
