Difference between revisions of "NusG"

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m (Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk)
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* '''Description:''' transcription antitermination and pausing factor <br/><br/>
  
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || transcription  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || transcription  
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01010 nusG]
 
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 5.252   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 5.252   
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|colspan="2" | '''Genetic context''' <br/> [[Image:nusG_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nusG_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nusG_117890_118423_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:nusG_expression.png|500px]]
 
 
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* '''Operon:'''  
 
* '''Operon:'''  
  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nusG_117890_118423_1 nusG] {{PubMed|22383849}}
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* '''[[Sigma factor]]:'''  
 
 
* '''Sigma factor:'''  
 
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
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* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =

Revision as of 10:28, 7 August 2012

  • Description: transcription antitermination and pausing factor

Gene name nusG
Synonyms
Essential no
Product transcription antitermination and pausing factor
Function transcription
MW, pI 19 kDa, 5.252
Gene length, protein length 531 bp, 177 aa
Immediate neighbours secE, rplK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NusG context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

transcription

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01010

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: KOW domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alexander V Yakhnin, Paul Babitzke
Mechanism of NusG-stimulated pausing, hairpin-dependent pause site selection and intrinsic termination at overlapping pause and termination sites in the Bacillus subtilis trp leader.
Mol Microbiol: 2010, 76(3);690-705
[PubMed:20384694] [WorldCat.org] [DOI] (I p)

Alexander V Yakhnin, Helen Yakhnin, Paul Babitzke
Function of the Bacillus subtilis transcription elongation factor NusG in hairpin-dependent RNA polymerase pausing in the trp leader.
Proc Natl Acad Sci U S A: 2008, 105(42);16131-6
[PubMed:18852477] [WorldCat.org] [DOI] (I p)

Geoff P Doherty, Donna H Meredith, Peter J Lewis
Subcellular partitioning of transcription factors in Bacillus subtilis.
J Bacteriol: 2006, 188(11);4101-10
[PubMed:16707701] [WorldCat.org] [DOI] (P p)

C J Ingham, P A Furneaux
Mutations in the ss subunit of the Bacillus subtilis RNA polymerase that confer both rifampicin resistance and hypersensitivity to NusG.
Microbiology (Reading): 2000, 146 Pt 12;3041-3049
[PubMed:11101662] [WorldCat.org] [DOI] (P p)

C J Ingham, J Dennis, P A Furneaux
Autogenous regulation of transcription termination factor Rho and the requirement for Nus factors in Bacillus subtilis.
Mol Microbiol: 1999, 31(2);651-63
[PubMed:10027981] [WorldCat.org] [DOI] (P p)