CotM

• Description: spore coat protein (outer)
  
  

Gene name	cotM
Synonyms	yneL
Essential	no
Product	spore coat protein (outer)
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotM
MW, pI	15 kDa, 3.942
Gene length, protein length	390 bp, 130 aa
Immediate neighbours	yneK, sspP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigK regulon, GerE regulon

The gene

Basic information

• Locus tag: BSU17970

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • spore coat PubMed

Database entries

• Structure:

• UniProt: Q45058

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotM PubMed

• Expression browser: cotM PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between yneK and cotM PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

S Reischl, S Thake, G Homuth, W Schumann
Transcriptional analysis of three Bacillus subtilis genes coding for proteins with the alpha-crystallin domain characteristic of small heat shock proteins.
FEMS Microbiol Lett: 2001, 194(1);99-103
[PubMed:11150673] [WorldCat.org] [DOI] (P p)

A O Henriques, B W Beall, C P Moran
CotM of Bacillus subtilis, a member of the alpha-crystallin family of stress proteins, is induced during development and participates in spore outer coat formation.
J Bacteriol: 1997, 179(6);1887-97
[PubMed:9068633] [WorldCat.org] [DOI] (P p)