DapA

• Description: dihydrodipicolinate synthase
  
  

Gene name	dapA
Synonyms
Essential	yes PubMed
Product	dihydrodipicolinate synthase
Function	biosynthesis of lysine and peptidoglycan
Gene expression levels in SubtiExpress: dapA
Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr
MW, pI	30 kDa, 4.928
Gene length, protein length	870 bp, 290 aa
Immediate neighbours	dapG, rnjB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins, essential genes

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU16770

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H₂O (according to Swiss-Prot)

• Protein family: DHDPS family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3CPR (from Corynebacterium glutamicum, 39% identity, 58% similarity) PubMed

• UniProt: Q04796

• KEGG entry: [3]

• E.C. number: 4.2.1.52

Expression and regulation

• Operon:
  • spoVFA-spoVFB-asd-dapG-dapA PubMed
  • asd-dapG-dapA PubMed

• Expression browser: dapA PubMed

• Sigma factor:
  • spoVFA: SigK PubMed
  • asd: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

R A Daniel, J Errington
Cloning, DNA sequence, functional analysis and transcriptional regulation of the genes encoding dipicolinic acid synthetase required for sporulation in Bacillus subtilis.
J Mol Biol: 1993, 232(2);468-83
[PubMed:8345520] [WorldCat.org] [DOI] (P p)

N Y Chen, S Q Jiang, D A Klein, H Paulus
Organization and nucleotide sequence of the Bacillus subtilis diaminopimelate operon, a cluster of genes encoding the first three enzymes of diaminopimelate synthesis and dipicolinate synthase.
J Biol Chem: 1993, 268(13);9448-65
[PubMed:8098035] [WorldCat.org] (P p)